[Lagerfeldt]

Sure it does.

Any unnecessary process will introduce more calculations and more potential errors and will in some cases require extra dithering too. if you take a dithered 16 bit file and drop the volume by -0.1 dB in you'll expand to 32 bit float (depending on your DAW) and bouncing directly to 16 bits again gives you a truncated signal. But adding dithering again will give to two layers of dithering on what is essentially still a 16 bit file. Not very nice.

Whether this has any effect on the actual subject of this thread I don't know.