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Any time you move a fader, normalise, pan, (other than hard left or right) use any plugin...pretty much do anything to audio inside a computer, there are rounding errors which distorts the signal.
Dont be paranoid about it, just use your ears and dont do unnessesary digital processing.
Having said that, who cares about distortion when everything gets clipped by 10db in mastering anyway.
M
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